Porcine Foot and Mouth disease Virus A Antibody (P.FMDV-A Ab) Test,
VETERINARY Porcine Range
Application:
The Porcine Foot and Mouth Disease Virus Anti-A Antibody (P.FMDV-A
Ab) Rapid Test Cassette (Serum/Plasma) is a lateral flow
immunochromatographic assay for the qualitative detection of
serotype-A FMDV antibodies in procine’s serum, plasma. It’s for the
monitoring of FMDV-A antibody level and providing an important
reference for the vaccination plan.
| Principle: | Chromatographic Immunoassay |
| Specimen: | S |
| Pack: | 10 T |
| Shelf Life: | 2 Years |
| Specificity: | 96.70% |
| Format: | Cassette |
| Reading Time: | 5-10 Minutes |
| Storage Temperature: | 4-30℃ |
| Sensitivity: | 97.50% |
| Accuracy: | 96.90% |
Description:
The P.FMDV-A Antibody Rapid Test Cassette (Serum/Plasma) is based
on sandwich lateral flow immunochromatographic assay. The test
cassette has a testing window for the observation of assay running
and result reading. The testing window has an invisible T (test)
line region and a C (control) line region before running the assay.
When the treated sample was applied into the sample well on the
cassette, the liquid will laterally flow through thesurface of the
test strip and react with the pre-coated FMDVA antigens. If there
are anti-A FMDV antibodies in the specimen, a visible T line will
appear in the T line region. If there are not anti-A FMDV
antibodies in the specimen, a visible T line will not appear in the
T line region. The C line should always appear after a sample is
applied, which indicates that proper volume of specimen has been
added and membrane wicking has occurred.
How to use?
Allow the test cassette, specimen, buffer, and/or controls to
equilibrate to room temperature (15-30 °C) prior to testing.
1. Collect swine’s fresh whole blood, and separate serum or plasma
from blood as soon as possible to avoid hemolysis. Only use clear,
non-hemolyzed specimens. Do not leave the specimens at room
temperature for prolonged periods. Serum and plasma specimens may
be stored at 2-8 °C for up to 3 days. For long term storage,
specimens should be kept below -20 °C.
2. Move the test cassette from the foil pouch and use it as soon as
possible. Best results will be obtained if the assay is performed
within one hour.
3. Place the test cassette on a clean and level surface. Hold the
dropper vertically and transfer 1 drop of sample(approximately
25μL) and immediately add 2 drops of buffer (approximately 40μL)
into the specimen well (S) of the test cassette, then start the
timer. See illustration below.
4. Read the results in 5-10 minutes. Do not interpret results after
15 minutes.
INTERPRETATION OF RESULTS
Positive: The presence of both C line and T line, no matter T line is clear
or vague.
Negative: Only C line appears.
Invalid: No colored line appears in C line region, no matter whether T line
appears.
