| Product Name: | Canine Distemper Virus + Canine Adenovirus-II Antigen Combo Rapid
Test Cassette (Secretions) |
| Principle: | Chromatographic Immunoassay |
| Specimen: | Secretions |
| Pack: | 10 T |
| Shelf Life: | 2 Years |
| Specificity: | 97.50% |
| Cat No: | VIDA-525 |
| Format: | Cassette |
| Reading Time: | 5-10 Minutes |
| Storage Temperature: | 2-30℃ |
| Sensitivity: | 97.96% |
| Accuracy: | 97.75% |
Canine Distemper Virus + Canine Adenovirus-II Antigen Combo Rapid
Test Cassette (Secretions)
INTENDED USE
CDV + CAV-II Antigen Combo Rapid Test Cassette (Secretions) is a
lateral flow immunochromatographic assay for the qualitative
detection of Canine Distemper Virus (CDV Ag) and canine
adenovirus-II antigen (CAV-II Ag) in dog’s nasal/ocular secretions.
PRINCIPLE
CDV+ CAV-II Antigen Combo Rapid Test Cassette is based on sandwich
lateral flow immunochromatographic assay. The test cassette has two
testing windows. Each testing window has an invisible T (test) zone
and C (control) zone. When sample is applied into the sample well
on the cassette, the liquid will laterally flow on the surface of
the test strip. If there is enough canine distemper virus and
adenovirus antigen in the sample, a visible T line will appear. The
C line should always appear after a sample is applied, indicating a
valid result. By this means, the cassette can accurately indicate
the presence of canine distemper virus and canine adenovirus
antigen in the sample.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or
refrigerated (2-30 °C). The test cassette is stable through the
expiration date printed on the sealed pouch. The test cassette must
remain in the sealed pouch until use. DO NOT FREEZE. Do not use
beyond the expiration date.
DIRECTIONS FOR USE
Allow the test cassette, specimen, buffer, and/or controls to
equilibrate to room temperature (15-30 °C) prior to testing.
1.Collect dog’s ocular and nasal secretions with the swab provided.
Make the swab wet sufficiently.
Note: Collect ocular secretion from upper and lower eyelids, please
avoid secretions at the corner of the eye.
2.Insert the wet swab into the plastic tubes with buffer. Agitate
it to assure good sample extraction.
3.Remove the test cassette from the foil pouch and place the test
cassette on a clean and level surface. Hold the dropper vertically
and transfer 3 drops of extracted sample (approximately 120 μl) to
the specimen well (S) of the test cassette, then start the timer.
See illustration below.
4.Read the result in 5-10 minutes. Do not interpret results after
15 minutes.
INTERPRETATION OF RESULTS
Positive: The presence of both C line and T line, regardless of T
line being strong or faint.Negative: Only clear C line appears.
Invalid: No colored line appears in C zone, regardless of T line’s
appearance.
LIMITATION
CDV+ CAV-II Antigen Combo Rapid Test Cassette is for in vitro
diagnostic use only for canines. All results should be considered
with other clinical information available from veterinarian. For
confirming the results, confirmatory methods such as PCR are
recommended.
